Sea cucumbers have long been purported as a source of traditional medicine including wound healing due to the presence of sulfated Glycosaminoglycans (GAGs). Total sulfated Glycosaminoglycan (GAG) from sea cucumber <I>Stichopus hermanni</I> and <I>Stichopus vastus</I> integumental tissue were investigated for evaluation of wound healing potential in rats. Three groups of female Sprague-Dawley rats each consist of 6 animals were assigned. Normal saline was applied as a control to group 1 rats while group 2 and group 3 rats were treated topically with total sulfated GAG from both sea cucumber species&#146; integumental tissue to 6 mm diameter of full thickness wound from day 0-12. The effects on the contraction rate of wound healing were assessed. Wounds treated with total sulfated GAG from <I>Stichopus hermanni</I> and <I>Stichopus vastus</I> integumental tissue showed significantly (p&lt;0.05) improve wound contraction rate compared to wounds in control group. These results seems to suggest the beneficial effects of total sulfated GAG particularly from the integument body wall of sea cucumber for the positive enhancement of wound healing process in rats model.