"One of the important effects of exercise on human body is on the metabolic system especially on lipids elevated lipids and lipoprotein are risk factors for coronary heart disease. This study was conducted to evaluate the lipid profile among Jordanian top athletes according to gender. The sample included top athletes (males and females) participated in different national teams. One hundred twenty eight Jordanian top athletes 67 males and 61 females mean age 18.6&plusmn;1 years with training experience at least 5 years and with a minimal training load of twenty training hours per week participated in competitive different sports (aerobic, anaerobic and aerobic-anaerobic) were included in this study. Group of healthy male and female (control group), matched for age and gender was also included (n = 90). No subject revealed evidences of cardiovascular disease, diabetes (fasting glucose &lt;7 mmol L<SUP>-1</SUP>) or hypertension  (blood pressure &lt;130/80 mm Hg) when tested by specialized physicians. The lipid pattern included cholesterol, triglycerides, HDL-cholesterol and LDL-cholesterol. The results showed n significant differences appeared between the males and females in control, aerobic and anaerobic groups over the lipid profile variables except for triglycerides but significant differences p&gt;0.05 appeared between the males and females groups in the aerobic-anaerobicgroup over the HDL-C and LDL. Researchers concluded that the most abnormalities observed on routine biochemical screening in male and female athletes are no clinical significance."