The aqueous extract of Ageratum conyzoides leaves in combination with honey were investigated for evaluation of their wound healing potential in rats. Three groups of male Sprague Dawley rats each consist of 6 animals. Rats were placed individually in cages and all animals were experimentally wounded in the posterior neck area. Pure honey was applied topically to wounds of Group 1 animals. Wounds of Group 2 rats were treated with Ageratum conyzoides leaves extract in combination with honey (100 mg leaf extract/ml of honey w/v), and solcoseryl ointment was applied topically to wounds of Group 3 animals as a reference. The effects of vehicles on the rate of wound infection and on the rate of wound healing were assessed. Wounds of all groups of animals showed clean and remain sterile throughout the experiment. Wounds treated with plant extract in combination with honey, and wounds treated with solcosery ointment significantly (p<0.05) accelerate wound healing potential compared to wounds treated with honey alone. These results strongly document the beneficial effects of plant extract for the acceleration of wound healing process and the rates of wounds sterility.