Somatic cells in milk from lactating women were used as a source of human DNA and as a substrate for Polymerase Chain Reaction (PCR). The DNA yield varies with the different stages of lactation as compared with that of peripheral blood leukocytes. Using primers targeting the human mitochondrial cytochrome-b gene, the PCR produced a 157 Base Pair (BP) PCR product from milk samples as well as from peripheral blood. However, amplification products were not detected when the PCR was applied to DNA from animal species including, sheep and cattle. The scientific data presented in this study indicated that DNA extracted from human milk could serve as substrates for direct sequencing of genes or a fragment of the human genome. Since collection of milk is a non invasive procedure, it can often substitute for blood as a source of DNA. The technical convenience of milk as a source of DNA can be expected to increase the field of application of marker-based methods for genetic analysis of the human genome.