Application of Nucleic Acid Amplification Technology for Detection of Rna Viruses: Current Status and Future Prospects

doi:ijmmas.2005.84.87

eISSN: 1994-4624 / ISSN: 1813-176x

International Journal of Molecular Medicine and Advance Sciences

2005, Volume 1, Issue 2 : 84-86 doi: ijmmas.2005.84.87

Research Article

Application of Nucleic Acid Amplification Technology for Detection of Rna Viruses: Current Status and Future Prospects

DOI : ijmmas.2005.84.87

Published

June 30, 2005

Abstract

The application of bioengineering to study the molecular epidemiology of viral diseases should facilitate rapid detection of an active infection. The surge of new techniques in cellular immunology and molecular biology has made possible the development of rapid, sensitive, specific and inexpensive assays for detection of RNA viruses using nucleic acid amplification. Because of the Ribonucleic acid (RNA) nature of the virus genome, a Reverse Transcription (RT) step involving synthesis of complementary DNA (cDNA) from RNA template would be necessary, before cyclic amplification with PCR. In this study, we evaluated the diagnostic potential of the Reverse Transcriptase (RT) polymerase chain reaction (RT-PCR)-based assays, recently developed in our laboratory, for rapid detection of viral infections in cell culture and clinical samples.

Keywords

Rna

Viruses

Molecular diagnoses

PCR

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

All papers should be submitted electronically. All submitted manuscripts must be original work that is not under submission at another journal or under consideration for publication in another form, such as a monograph or chapter of a book. Authors of submitted papers are obligated not to submit their paper for publication elsewhere until an editorial decision is rendered on their submission. Further, authors of accepted papers are prohibited from publishing the results in other publications that appear before the paper is published in the Journal unless they receive approval for doing so from the Editor-In-Chief.

Int. J. Mol. Med. Adv. Sci. open access articles are licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets the audience to give appropriate credit, provide a link to the license, and indicate if changes were made and if they remix, transform, or build upon the material, they must distribute contributions under the same license as the original.

Recommended Articles

Clinical Efficacy of Drug-Eluting Stents Among Hypertensive Patients

Arif Mohammad,

Sophia Menon,

Carlos E. Martinez

41-45

Occupational Health Risks Among Healthcare Workers: Prevalence, Determinants, Clinical Consequences, and Prevention Strategies

Jennifer L. Roberts,

Ahmed M. Hassan,

Priyanka S. Nair

1-4

Healthcare Disparities and Equity in Medical Services: Challenges, Determinants, and Strategies for Achieving Equitable Healthcare